License Statement
| ID Number | |
|---|---|
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq Single Cell Kit isdesigned togenerate high-quality, full-length cDNA directly from single cells.It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells).This kit supports up to 12 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components You May Also Like Image Data
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634470: SMART-Seq Single Cell Kit
Back
The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.
Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.
Back
Automation and miniaturization of cDNA synthesis on the mosquito HV.
Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.
Back
Automation and miniaturization of SMART-Seq mRNA Single Cell on the MANTIS Liquid Dispenser.
Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS Liquid Dispenser. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.
Back
Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.
Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles.Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.
License Statement
| ID Number | |
|---|---|
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells). This kit supports up to 48 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components You May Also Like Image Data
Back
634471: SMART-Seq Single Cell Kit
Back
The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.
Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.
Back
Automation and miniaturization of cDNA synthesis on the mosquito HV.
Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.
Back
Automation and miniaturization of SMART-Seq mRNA Single Cell on the MANTIS Liquid Dispenser.
Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS Liquid Dispenser. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.
Back
Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.
Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles.Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.
License Statement
| ID Number | |
|---|---|
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells). This kit supports up to 96 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components You May Also Like Image Data
Back
634472: SMART-Seq Single Cell Kit
Back
The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.
Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.
Back
Automation and miniaturization of cDNA synthesis on the mosquito HV.
Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.
Back
Automation and miniaturization of SMART-Seq mRNA Single Cell on the MANTIS Liquid Dispenser.
Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS Liquid Dispenser. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.
Back
Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.
Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles.Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.
License Statement
| ID Number | |
|---|---|
| 385 | This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com. |
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq Single Cell PLUS Kit is a complete kit designed to first generate high-quality cDNA from single cells and then high-quality Illumina sequencing-ready libraries. Indexes are added using a unique dual index kit (Cat. # R400744 or R400745). This kit supports up to 48 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components Image Data
Back
The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.
Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.
Back
Automation and miniaturization of cDNA synthesis on the mosquito HV.
Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.
Back
Automation and miniaturization of SMART-Seq mRNA Single Cell on the MANTIS Liquid Dispenser.
Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS Liquid Dispenser. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.
Back
Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.
Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.Representative XY-plots of two example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate, and the same cDNA library compared between Nextera XT and PLUS. R2 values are shown in the graph; Nextera XT R2=0.9961, PLUS R2=0.9978, Nextera XT vs PLUS R2=0.9579.Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.
Back
Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.
Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles.Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.
Back
R400750: SMART-Seq Single Cell PLUS Kit
License Statement
| ID Number | |
|---|---|
| 385 | This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com. |
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq Single Cell PLUS Kit is a complete kit designed to first generate high-quality cDNA from single cells and then high-quality Illumina sequencing-ready libraries. Indexes are added using a unique dual index kit (Cat. # R400744 or R400745). This kit supports up to 96 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components Image Data
Back
The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.
Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.
Back
Automation and miniaturization of cDNA synthesis on the mosquito HV.
Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.
Back
Automation and miniaturization of SMART-Seq mRNA Single Cell on the MANTIS Liquid Dispenser.
Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS Liquid Dispenser. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.
Back
Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.
Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.Representative XY-plots of two example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate, and the same cDNA library compared between Nextera XT and PLUS. R2 values are shown in the graph; Nextera XT R2=0.9961, PLUS R2=0.9978, Nextera XT vs PLUS R2=0.9579.Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.
Back
Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.
Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles.Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.
Back
R400751: SMART-Seq Single Cell PLUS Kit
